goat anti chicken igm antibody (Bethyl)

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Bethyl goat anti chicken igm antibody
Goat Anti Chicken Igm Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chicken+igm/Chicken+IgM+Antibody/pm41887171-122-12-24
Average 93 stars, based on 205 article reviews

goat anti chicken igm antibody - by Bioz Stars, 2026-09

93/100 stars

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Expressing:

Article Title: Monoclonal antibody generation by controlled immunoglobulin gene rearrangements.
Article Snippet: .. To examine the expression level of chicken IgM, cells (1 × 106) were washed twice with FACS buffer (PBS containing 0.5% bovine serum albumin and 2mM EDTA) and stained with FITC labeled anti-chicken IgM antibody (Bethyl, catalog no. A30-102F, 1:250 dilution) for 15m. .. Cells were washed twice by FACS buffer and analyzed by NovoCyte (Agilent, CA, USA) or SH800.

Article Title: Monoclonal antibody generation by controlled immunoglobulin gene rearrangements
Article Snippet: .. To examine the expression level of chicken IgM, cells (1 × 10 6 ) were washed twice with FACS buffer (PBS containing 0.5% bovine serum albumin and 2 mM EDTA) and stained with FITC labeled anti-chicken IgM antibody (Bethyl, catalog no. A30-102F, 1:250 dilution) for 15 m. Cells were washed twice by FACS buffer and analyzed by NovoCyte (Agilent, CA, USA) or SH800. .. For the detection of chicken Igλ, unlabeled mouse anti-chicken Igλ monoclonal antibody (Southern Biotech, AL, USA, catalog no. 8340-01) was labeled with biotin using Biotin Labeling Kit-NH 2 (Dojindo, Kumamoto, Japan, catalog no. LK03) and used identically to anti-chicken IgM antibody except that FITC-labeled streptavidin (BioLegend, 1:1000 dilution) was added.

FACS:

Staining:

Article Title: Regulation of B cell differentiation by the ubiquitin-binding protein TAX1BP1
Article Snippet: Single-cell suspensions of total splenocytes from WT and TAX1BP1 −/− mouse were stained with different combinations of the following antibodies: PE/Cy7 anti-TCR β chain, PerCP-Cy5.5 anti-Gr-1, 7-AAD, FITC anti-mouse IgM, APC anti-CD23, PE anti-CD21 (BioLegend), or PerCP-Cy5.5 anti-Gr-1, 7-AAD, PE/Cy7 anti-B220, PE anti-CD93, FITC anti-mouse IgM and APC-CD23 (BioLegend). .. WT DT40 cells, TAX1BP1 −/−/− cells and TAX1BP1 −/−/− /Tax1bp1 cells were stained with FITC conjugated anti chicken IgM (Bethyl). .. FACS Verse (BD) and SH800 (Sony Biotechnology Inc.) were used for flow cytometry, and results were analyzed with FlowJo software.

Labeling:

Incubation:

Article Title: Immunoglobulin knockout chickens via efficient homologous recombination in primordial germ cells.
Article Snippet: To measure total plasma IgM and IgY, enzyme immunoassay (EIA) plates (Corning) were coated with 2 μg/mL goat anti–chicken-IgM (Bethyl Laboratories) or rabbit anti–chicken-IgY (Sigma Aldrich) overnight. .. Plates were blocked with 3% (wt/vol) skim milk and incubated with chicken plasma for 2 h. Bound chicken IgM or IgY was detected with goat anti–chicken-IgMHRP (Bethyl Laboratories) or rabbit anti–chicken-IgY-HRP (Sigma Aldrich) and developed with 3,3′,5,5′-tetramethylbenzidine (TMB) substrate. ..

Clinical Proteomics:

Article Title: Long-term effects of early life microbiota disturbance on adaptive immunity in laying hens.
Article Snippet: .. Total Ig plasma concentrations were determined on d 35, 70, 105, 140, and 175 by means of chicken IgM and IgY ELISA quantitation sets according to manufacturer’s recommendations (Bethyl Laboratories, Inc, Montgomery, TX, catalog E30–102 and E30–104, respectively). ..

Enzyme-linked Immunosorbent Assay:

Article Title: Regulation of B cell differentiation by the ubiquitin-binding protein TAX1BP1
Article Snippet: Concentrations of antibodies specific for SRBCs were determined by ELISA using the mouse anti-SRBC ELISA kit (Life Diagnostics, Inc.), according to the manufacturer’s instructions. .. Concentrations of chicken IgM were determined by ELISA using chicken IgM ElISA kit (Bethyl Laboratories, Inc), according to the manufacturer’s instructions. .. Total RNA was extracted from the DT40 cell lines and isolated splenic B cells from unimmunized or SRBC-immunized mice using ReliaPrep RNA Cell Miniprep System (Promega) and used as a template to create cDNA by ReverTra Ace qPCR RT kit (TOYOBO).

Article Title: Evaluation of humoral immune response, body weight and blood constituents of broilers supplemented with phytase on infectious bursal disease vaccination
Article Snippet: The Specific Ab to IBDV was detected by using Infectious Bursal Disease Virus Antibody Test kit (FlockChek®, IDEXX Laboratories, USA). .. Chicken IgM, Chicken IgA and Chicken IgG ELISA Quantitation Set (Bethyl Laboratories, Inc., USA) were also used to determine non-specific of IgM, IgA and IgG, respectively. ..

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